Molecular Biology Services

MOLECULAR BIOLOGY SERVICES

Vector construction · Mutagenesis · Full-length gene synthesis · shRNA / miRNA tools
Efficient, reliable and customizable molecular biology services for research

PromoterGOIPlasmid
Vector
OriTerminator

01 Service Overview

Our molecular biology services cover vector construction, mutagenesis, full-length gene synthesis, RNA tool vector construction and plasmid preparation for diverse research projects.

Vector Construction

For inserts of various sizes
Expression vector construction

Gene mutagenesis

Site-directed mutagenesis and
diverse molecular modifications

RNA tool construction

shRNA, inducible shRNA,
pre-miRNA and inhibitor vectors

Plasmid preparation

Endotoxin-free plasmid
large-scale preparation

02 Our molecular biology services

Vector construction / gene synthesis

  • Vector construction for <3 kb, 3–5 kb and 5–7 kb inserts
  • Full-length gene synthesis <3 kb
  • For conventional expression vectors and functional studies
Promoter GOI Vector

Gene mutagenesis

  • Mutagenesis of regions <10 bp
  • Mutagenesis of regions 10–20 bp
  • For functional validation and site-specific studies
Template Mutation Validation

shRNA / inducible shRNA
Vector Construction

  • Construction of 1, 2 or 3 shRNA vectors
  • Co-expression of 2 / 4 shRNAs from one vector
  • Inducible shRNA design and construction
U6 / H1 shRNA Terminator

miRNA and large-scale plasmid preparation

  • pre-miRNA expression vector construction
  • microRNA inhibitor expression vector construction
  • Endotoxin-free large-scale plasmid extraction
miRNA / / inhibitor Plasmid Prep

03 Services and deliverables

Molecular biology services 1–10
Service code Service Turnaround (business days) Specification
TS1001 Vector construction, gene size <3 kb 105ug
TS1002 Vector construction, gene size 3–5 kb 105ug
TS1003 Vector construction, gene size 5–7 kb 105ug
TS1004 Gene mutagenesis, region <10 bp 105ug
TS1005 Gene mutagenesis, region 10–20 bp 105ug
TS1006 Full-length gene synthesis <3 kb 5~155ug
TS1007 Construction of 1 shRNA expression vector 105ug
TS1008 Construction of 2 shRNA expression vectors 105ug
TS1009 Construction of 3 shRNA expression vectors 105ug
TS1010 One vector co-expressing 2 shRNAs (2 different promoters) 155ug
Molecular biology services 11–20
Service code Service Turnaround (business days) Specification
TS1011 One vector co-expressing 4 shRNAs (4 different promoters) 205ug
TS1012 Construction of 1 inducible shRNA expression vector 105ug
TS1013 Construction of 2 inducible shRNA expression vectors 105ug
TS1014 Construction of 3 inducible shRNA expression vectors 105ug
TS1015 One vector co-expressing 2 inducible shRNAs 155ug
TS1016 One vector co-expressing 4 inducible shRNAs 205ug
TS1017 microRNA precursor (pre-miRNA) expression vector construction 105ug
TS1018 microRNA inhibitor expression vector construction 105ug
TS1019 Large-scale plasmid extraction (endotoxin-free) 5100ug
TS1020 Large-scale plasmid extraction (endotoxin-free) 101mg

Contact a technical advisor for additional customization. We will tailor the proposal to your project.

04 Service Process

A standardized service workflow to advance your research efficiently.

  1. 01

    Project Consultation

    Clarify project objectives
    and technical requirements

  2. 02

    Scope Confirmation

    Confirm the construction strategy
    and delivery plan

  3. 03

    Design and construction

    Vector design,
    mutagenesis or synthesis

  4. 04

    Quality verification

    Sequence confirmation and
    gene quality control

  5. 05

    Project Delivery

    Deliver plasmids and
    associated results

05 Tell Us About Your Research

Complete the form below. We will contact you soon with technical support and advice.

Service type *

We will contact you within 1 business day with technical support and advice.

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WeChat Official Account

Original Ephraim Lifescience WeChat official account QR code 13959265142 (also on WeChat) David@ephraim.org.cn

CONTACT EPHRAIM

Discuss your project with our team

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Tell us about your research goals and service needs.

WeChat

WeChat: 13959265142

EPHRAIM LIFESCIENCE (XIAMEN) CO., LTD.

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You can also contact us directly by email or telephone.