Stable expression
Stable expression over long-term passaging
01 Service Overview
Stable cell line development introduces genetic material into host cells to achieve long-term target gene expression or knockdown. Applications include gene function studies, protein expression, drug screening, mechanistic validation and cell model development.
Overexpression / shRNA knockdown
For diverse research needs
Adherent / Suspension cells
Compatible with diverse cell types
Polyclonal / Monoclonal delivery
Customized options to suit your needs
02 Our service options
We offer stable overexpression and gene knockdown cell line development tailored to your research goals.
Stable overexpression cell lines
- Stable integration of the target gene into the cellular genome
- For functional studies, protein analysis, drug screening and more
- Multiple promoters and vector systems supported
Promoter
GOI
Selection
Stable knockdown cell lines （shRNA）
- Cell lines stably expressing shRNA targeting the gene of interest
- For gene function validation, signaling pathway studies and more
- Choice of vector systems and selection markers
U6 / H1
shRNA
Terminator
Selection
03 Choose a service plan
Select a service to jump to the inquiry form below
CELL TYPE
Adherent cells — overexpression / knockdown
Suitable for most common adherent cells, including:
HEK293, HeLa, NIH3T3, CHO, A549 and more
CELL TYPE
Suspension cells — overexpression / knockdown
Suitable for suspension cells, including:
Jurkat, K562, 293F, CHO-S and more
DELIVERY TYPE
Delivery options — polyclonal / monoclonal
Polyclonal: shorter turnaround, suitable for most studies
Monoclonal: higher cell population purity and more uniform expression
04 Service Process
- 01
Project Consultation
Understand the research objective
Define the experimental plan - 02
Vector Construction
Construct an overexpression or
shRNA vector - 03
Gene delivery and selection
Optimize delivery conditions
Obtain positive cells - 04
Single-clone selection (optional)
Select single clones as needed and
verify gene expression and phenotype - 05
Stability verification and delivery
Verify stability over multiple passages
Deliver cell lines and test reports
Quality control
- Cell condition assessment
- Expression / knockdown verification
- Stability assessment
- Project report
05 Tell Us About Your Research
Please complete the information below. We will contact you promptly to discuss a suitable experimental plan.